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Zi-Guo Yuan

Zi-Guo Yuan

 Discipline:	Parasitology, Preventive Veterinary Medicine
Tel\Fax:	+86-13826436231
E-Mail:	ziguoyuan@scau.edu.cn
Address:	College of Veterinary Medicine, South China Agricultural University, 510642
Additional Information:	Toxoplasma Gondii

Resume:

2005-2008, Ph.D thesis: Construction of Recombinant Pseudorabies Virus Expressing Rabies Virus Glycoprotein and Research of Immunity Test. We have used it to construct a recombinant virus, rPRV/eGFP/rgp, expressing the rabies virus glycoprotein using Pseudorabies virus, which has been licensed for use as a live vaccine in pigs and possesses an excellent safety and efficacy record. This recombinant virus has been shown to be safe for dogs by oral and intramuscular routes of inoculation and was demonstrated to induce immune responses against both pseudorabies and rabies in dogs after a single oral dose of 2 × 107.0 plaque forming units (PFU). Neutralizing antibody titers against rabies reached >0.5 IU/ml and 1:64—1:128 against pseudorabies by 5 weeks post-vaccination in all dogs, indicating that the pseudorabies virus vector infected dogs and replicated in vivo, and that the rabies virus glycoprotein had been expressed and an effective immune response elicited. Anti-body titers were maintained for over 6 months. This suggests that pseudorabies virus could be an effective live vector for recombinant rabies oral vaccination.  Results were published in Vaccine.

I have 10 years of research experience in Parasitology and 4 years for Rabies and Canine Influenza Virus. I graduated in Jun 2008, and worked in department of preventive veterinary, South China Agricultural University. In May, 2011, we went to the lab charged in associated professor Cunlei Su to study technology related to T.gondii, University of Tennessee.

My research focus has been predominantly molecular and immunological parasitology. My scientific output to date consists of 2 books (in Chinese); more than 78 papers in English in well-regarded international journals (SCI cited) and more than 30 papers in refereed national journals. I have brought 3 research students for PhD degree, 9 research students for MSc degree and 27 research students for Bachelor to completion, and I am now supervising 2 associate professors.

Mastering of experimental techniques:

Molecular biology: DNA and RNA isolation and purification, DNA cloning, library construction and screening, Suppression subtractive hybridization (SSH), Southern blotting, Northern blotting, agarose gel electrophoresis,  PCR; long PCR; Cycle DNA sequencing, Random amplified polymorphic DNA (RAPD)-PCR, PCR-linked restriction fragment length polymorphism (PCR-RFLP), PCR-based single strand conformation polymorphism (PCR-SSCP), Simple sequence repeat-PCR (SSP-PCR), Rapid amplification of cDNA ends (RACE), RNA interference (RNAi), microRNA technology, etc.

Immunology: Preparation and purification of parasite antigens for application in immunoassay and immunization trials; establishment, standardization and quality control of immunoassay; establishments of ELISA, Dot-ELISA, immuohistochemical staining techniques, Immunofluorescence assay (IFA), immunoblot (Western Blot), immunization protocols, SDS-PAGE and isoelectric focusing electrophoresis (IEF); vaccine development; FACS analysis of blood lymphocyte phenotypes; in vitro cultivation of blood/mesenteric lymphocytes for mitogenic responses, antigenic responses and cytokine production.

Research Interests:

Functional genomics, Immunology of parasites; molecular cloning and expression of functional genes of parasites; vaccine development; genetics and molecular diagnosis of parasitic diseases; PCR-based technology in parasitology; epidemiology and control strategies for parasitic infections; Proteomics and microRNA of T.gondii.

But, the current research directions: the invasion mechanism of Toxoplasma gondii breaking through the blood-brain barrier; and the mechanism of Toxoplasma gondii causing reproductive dysfunction.

Grants: 

1. National Key R&D Program Project, 2018YFD0501205, Integrated and Demonstration of Comprehensive Prevention and Control Technology for Large-scale Local Pig Breeding Diseases, 2018/06-2020/12, 1,840,000 RMB, PI

2. Sub-project of National Key R&D Program, 2017YFD0501305, rapid detection, treatment and carrier vaccine construction of Cryptosporidium, 2017/06-2020/12, 450,000 RMB, PI

3. Guangdong Provincial Natural Science Foundation Project, 2016A030313396, MicroRNA-mediated study of Toxoplasma gondii bark protein evading host immune mechanism, 2016/06-2019/06, 100,000 RMB, PIAwards, honours, scholarships and fellowships:

(1) Research project entitled Research and application of key technologies for prevention and control of important zoonotic zoonosis was awarded the first prize of Guangdong Science and Technology Award in 2016, P.R.China, Ranking 7.(Certeficate No. [2016]3586)

(2) Research project entitled “Localization and immune function of ROP16 gene of Toxoplasma gondii”  was awarded the second prize of Excellent academic report of the thirteenth Symposium of animal husbandry and Veterinary Association of China in 2015.

(3) Research project entitled “Innovation and application of detection technology for major epidemic diseases in dairy cattle” was awarded the second prize of Guangdong  natual science award , P.R.China, Ranking 6. (Certeficate No. [2014]1269)

(4)  Research project entitled “Rapid detection and diagnosis and treatment technology of dairy cow mastitis” was awarded the first prize in Science and Technology Promotion Award, P.R.China, Ranking 6. (Certeficate No.2012108)

(5)  Cultivated Talents by “Thousand-Hundred-Ten” Program of Guangdong Province, Guangdong Province, P.R. China in 2014

(6)  Research project entitled The researches of zoonotic parasitic etiology, molecular genetics and functional genomics was awarded the first prize by the Ministry of Education, P.R. China in 2011, Ranking 6. (Certeficate No.2010-044)

(7) First session of “New Star of Pearl River” Distinguished Professorship awarded by the   Department of Science & Technology, Guangzhou City, Guangdong Province, P.R.China, 2011

(8) The honorary title “The Second Prize of Speech Match” awarded by College of Veterinary Medicine, South China Agricultural University in 2010.

(9) The honorary title “The Outstanding Teacher of Class” awarded by College of Veterinary Medicine, South China Agricultural University in 2010.

(10)  The honorary title “The Associate Professor of Important Cultivation” awarded by College of Veterinary Medicine, South China Agricultural University in 2010.

(11) Cultivated Talents by the Seventh Personnel Training of the Project of “Thousand-Hundred-Ten” Program of Guangdong Province, Guangdong Province, P.R. China in 2014

(12) Paper entitled Studies on immunogenicity of Toxoplasma gondii MIC6 antigen won the Second Prize for Excellent Scientific Papers, awarded by Department of Education, Guangdong Provincial Government in 2011.

(13) The honorary title “Excellent Class Teacher” awarded by College of Veterinary Medicine, South China Agricultural University in 2011.

(14) Honorary Certificate issued by ACS Publications for excellence in information contribution in 2011.

(15) The Speaker Teacher of National Bilingual Education Model Curriculum-Small Animals of Parasitic Diseases, awarded by the Ministry of Education, 2010, China

(16) The curriculum of Small Animals of Parasitic Diseases (I am in charge of the course) was chosen by 2011 Guangdong Higher quality courses, Department of Education, Guangdong Province, China

(17) Distinguished Professorship awarded by the Department of Science & Technology, Guangzhou City, Guangdong Province, China in 2008. 

puPublications:(*Corresponding Author)

(1) Lin Lv, Yapei Wang, Weili Feng, Jorge A. Hernandezc, Wanyi Huang, Yuxiang Zheng, Xue Zhou, Shumei Lv, Yajun Chen, Zi-Guo Yuan*, iTRAQ-based differential proteomic analysis in Mongolian gerbil brains chronically infected with Toxoplasma gondii. Journal of Proteomics, 2017, 160:74-83. (IF:3.722)

(2) Xiu-Zhen Li#, Lin Lv#, Xu Zhang#, Kenneth Yongabi Anchang, Auwalu Yusuf Abdullahi, Liqing Tu, Xiaohu Wang, Lijun Xia, Xiu-Xiang Zhang, Weili Feng, Chunni Lu, Shou-Jun Li*, Zi-Guo Yuan*, Recombinant canine adenovirus type-2 expressing TgROP16 provides partial protection against acute Toxoplasma gondii infection in mice. Infect Genet Evol, 2016, 45:447-453.(IF:2.885)

(3) Xiu-Zhen Li#, Xiao-Hu Wang#, Li-Jun Xia, Ya-Biao Weng, Jorge A Hernandez, Li-Qing Tu, Lu-Tao Li, Shou-Jun Li, Zi-Guo Yuan*, Protective efficacy of recombinant canine adenovirus type-2 expressing TgROP18 (CAV-2-ROP18) against acute and chronic Toxoplasma gondii infection in mice, BMC Infectious Diseases, 2015,15(1): 114. (IF:2.69)

(4) Xiu-Xiang Zhang#, Hui Yu#, Xiao-Hu Wang#, Xiu-Zhen Li, Yan-Ping Zhu, Hao-Xin Li, Sheng-Jun Luo*, Zi-Guo Yuan*, Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice, Vaccine, 2013, 31(4):698-703. (IF:3.413)

(5) Zi-Guo Yuan#*, Di Ren#, Dong-Hui Zhou, Xiu-Xiang Zhang, Eskild Petersen, Xiu-Zhen Li, Yang Zhou, Gui-Lian Yang, Xing-Quan Zhu*, Evaluation of protective effect of pVAX-TgMIC13 plasmid against acute and chronic Toxoplasma gondii infection in a murine model, Vaccine, 2013, 31(31): 3135-9. (IF:3.413)

(6) Zi-Guo Yuan#, Xiu-Xiang Zhang#, Rui-Qing Lin, Eskild Petersen, Shenyi He, Miao Yu, Xian-Hui He, Dong-Hui Zhou, Yong He, Hao-Xin Li, Ming Liao*, Xing-Quan Zhu*, Protective effect against toxoplasmosis in mice induced by DNA immunization with gene encoding Toxoplasma gondii ROP18, Vaccine, 2011, 29(38): 6614-9. (IF:3.413)

(7) Zi-Guo Yuan#, Xiu-Xiang Zhang#, Xian-Hui He, Eskild Petersen, Dong-Hui Zhou, Yong He, Rui-Qing Lin, Xiu-Zhen Li, Xu-Li Chen, Xiao-Ru Shi, Xiu-Ling Zhong, Bing Zhang, Xing-Quan Zhu*, Protective immunity induced by Toxoplasma gondii rhoptry protein 16 against toxoplasmosis in mice, Clin Vaccine Immunol, 2011, 18(1): 119-24. (IF:2.872)

(8) Zi-Guo Yuan#, Sheng-Jun Luo#, Hui-Juan Xu, Xiao-Hu Wang, Juan Li, Li-Guo Yuan, Le-Tian He, Xiu-Xiang Zhang*, Generation of E3-deleted canine adenovirus type 2 expressing the Gc glycoprotein of Seoul virus by gene insertion or deletion of related terminal region sequences, J Gen Virol, 2010, 1764-71. (IF:3.192)

(9) Zi-Guo Yuan#, Xiu-Ming Li#, Yasser Saad Mahmmod, Xiao-Hu Wang, Hui-Juan Xu, Xiu-Xiang Zhang*, A single immunization with a recombinant canine adenovirus type 2 expressing the seoul virus Gn glycoprotein confers protective immunity against seoul virus in mice, Vaccine, 2009, 27(38): 5247-51. (IF:3.413)

(10) Zi-Guo Yuan, Shoufeng Zhang, Ye Liu, Fei Zhang, Anthony R. Fooks, Qianxue Li, Rongliang Hu*, A recombinant pseudorabies virus expressing rabies virus glycoprotein: safety and immunogenicity in dogs, Vaccine, 2008, 26(10): 1314-21.  (IF:3.413)

(11) Chen Y, Yu M, Hemandez JA, Li J, Yuan Zi-Guo*, Yan Hai-Kuo. Immuno-efficacy of DNA vaccines encoding PLP1 and ROP18 against experimental Toxoplasma gondii infection in mice. Exp Parasitol. 2018 May;188:73-78. (IF:1.821)

(12) Tu L, Zhou P, Li L, Li X, Hu R, Jia K, Sun L, Yuan Ziguo*, Li S. Evaluation of protective efficacy of three novel H3N2 canine influenza vaccines. Oncotarget. 2017 Sep 20;8(58):98084-98093. (IF:5.168)

(13) Yuan Ziguo, Gao S, Liu Q, Xia X, Liu X, Liu B, Hu R. Toxoplasma gondii antibodies in cancer patients. Cancer Lett. 2007 Aug 28;254(1):71-4. (IF:6.491)

(14) Yuan Ziguo, Zhang X, Zhang S, Liu Y, Gao S, Zhang F, Xu H, Wang X, Hu R. Development of recombinant canine adenovirus type-2 expressing the Gn glycoprotein of Seoul virus. Biologicals. 2008 May;36(3):162-7. (IF:1.872)

(15) Yuan Ziguo, Zhang S, Liu Y, Zhang F, Fooks AR, Li Q, Hu R. A recombinant pseudorabies virus expressing rabies virus glycoprotein: safety and immunogenicity in dogs. Vaccine. 2008 Mar 4;26(10):1314-21. (IF:3.413)

(16) Liu GH, Zhang LX, Zou FC, Yuan Zi-Guo, Zhao GH, Hu M, Suo X, Zhu XQ. Veterinary parasitology teaching in China in the 21st century - Challenges, opportunities and perspectives. Vet Parasitol. 2018 Mar 15;252:70-73. (IF:2.422)

(17) Fu C, Luo J, Ye S, Yuan Zi-Guo, Li S. Integrated Lung and Tracheal mRNA-Seq and miRNA-Seq Analysis of Dogs with an Avian-Like H5N1 Canine Influenza Virus Infection. Front Microbiol. 2018 Mar 5;9:303. (IF:4.019)

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